BD Vacutainer CPT單個核細胞制備管提供了一種快速分離濃縮血小板（PRP）的方法，一次離心（zui多半小時），即可得到PRP?？梢宰鳛镻RP美容采血管。

BD Vacutainer CPT 采血管

產(chǎn)品訂購信息

———————————————BD 362761現(xiàn)貨直達————————————————

PRP美容采血管自體血清制備BD Vacutainer CPT Mononuclear Cell Preparation Tube 362761單個核細胞準備管

歡迎您致電 華雅再生醫(yī)學旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司  ：1500 1904 520。紅榮微再-客服: 2395557778  經(jīng)銷商專員

BD 362761采血管參數(shù)

REF 362761

8 mL Draw Capacity (16 x 125 mm Tube Size) Sterile Tube Interior

表面材質(zhì)

Silicone Coated Glass Tube

Silicone Lubricated Rubber Stopper

密度梯度原理：FICOLL法

抗凝劑1.0 mL of 0.1 Molar Sodium Citrate Solution (Top Fluid Layer)

分離膠

3.0 gm of Polyester Gel (Middle Layer)

2.0 mL of Polysaccharide/Sodium Diatrizoate Solution (FICOLL™ Hypaque™ solution, Bottom Fluid Layer)

離心 20 minutes at 1500 to 1800 RCF。zui高30min。

分析

Recovery and Purity percentages：

Coulter Counter® Model S PLUS IV

Viability percentages：

Acridine Orange/Ethidium Bromide staining

Red blood cell percentages：

Hemocytometer count under a light microscope

BD Vacutainer CPT采血管系列參考資料：

GENERAL REFERENCES

Centers for Disease Control. Recommendations for Prevention of HIV Transmission in Health-Care Settings. MMWR 1987, 36 (suppl.no. 2S), pp. 3S - 17S.

Centers for Disease Control. Update: Universal Precautions for Prevention of Transmission of Human Immunodeficiency Virus,Hepatitis B Virus, and Other Bloodborne Pathogens in Health-Care Settings. MMWR 1988, 37 No. 24, pg. 380.

National Committee for Clinical Laboratory Standards. Protection of Laboratory Workers from Infectious Disease Transmitted by Blood Tissue. Tentative Guidelines NCCLS Document M29-T.NCCLS; 1991, Villanova, PA.

OSHA Final Standard for Occupational Exposure to Bloodborne Pathogens. 56 Fed. Reg. 64 175, Dec. 6, 1991; 29 CFR Part 1910.1030.

REFERENCES

1. Boyum, A. Isolation of mononuclear cells and granulocytes from human blood. Scand. J.Clin. Lab. Invest. 21, Suppl 97 (Paper IV), 77-89, 1968.

單個核細胞分離

2. Ting, A. and Morris, P.J. A technique for lymphocyte preparation from stored heparinized blood. Vox. Sang. 20:561-563,1971.

肝素血制備淋巴細胞

3. Fotino, M., Merson, E.J. and Allen, F.H. Micromethod for rapid separation of lymphocytes from peripheral blood. Ann. Clin. Lab. Sci. 1:131-133, 1971.

淋巴細胞快速分離

4. Dwyer, J.M., Finklestein, F.O., Mangi, R.J., Fisher, K. and Hendler, E. Assessment of the adequacy of immunosuppressive therapy using microscopy techniques to study immunologic competence. Transplant. Proc. 7:785, 1975.

5. Brown, G. and Greaves, M.F. Enumeration of absolute numbers of T and B cells in human blood. Scand. J. Immunol. 3:161, 1974.

T細胞和B細胞計數(shù)

6. McCarthy, D.A., Perry, J.D., et.al. Centrifugation of Normal and Rheumatoid Arthritis Blood on FICOLL™-Hypaque™ and FICOLL™-Nycodenz Solution. J. Immunol. Meth. 73:415-425 (1984).

分離液FICOLL的離心

7. Data on File, Report No. R-88-99-QC-195, BD Vacutainer Systems, NJ

8. Needham, P.L. Separation of human blood using "Mono- Poly Resolving Medium." J. Immunol. Meth. 99:283, 1987

PRP介紹

PRP為Plaet-rich plasma的縮寫，中文為“高濃度血小板血漿”，PRP具有迅速止血、止痛、加速傷口愈合的作用。PRP的意思是富血小板血漿，PRP血清因子更是明星永葆青春的秘密，PRP自體血清注射回春術(shù)用自身血液制作出富含高濃度血小板和自身生長因子的血漿，重新注射到組織中，PRP自體血清注射回春術(shù)提高皮膚內(nèi)膠原蛋白和彈力纖維的含量。利用少量自身血液用高科技分離技術(shù)，使用BD362761/362760/362753系列PRP采血管，可分離出含高濃度細胞生長因子的富血小板血清“Plaet-Rich Plasma”，簡稱PRP。然后再注入肌膚，其活性PRP內(nèi)里蘊藏多種皮層生長及修復因子，能修補衰老受損肌膚，激活皮膚細胞功能，改善新陳代謝，讓肌膚注滿能量并得以再生，延緩衰老。接受ACR-PRP激活肌膚療程后，一般于數(shù)周至一個月骨可見肌膚質(zhì)素有多方面的改善，包括彈性、光澤度、緊致度和嫩滑程度等，讓肌膚煥發(fā)青春神采。

———————————————BD 362761采血管現(xiàn)貨直達—————————————

PRP美容采血管自體血清制備BD Vacutainer CPT Mononuclear Cell Preparation Tube 362761單個核細胞準備管

歡迎您致電 華雅再生醫(yī)學旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司  ：1500 1904 520。紅榮微再-客服: 2395557778  經(jīng)銷商專員